F1ANNEX IITEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.
Annotations:
Amendments (Textual)
Appendix 6Validated PCR protocols and reagents
3.Multiplex PCR protocol with internal PCR control (Pastrik et al., 2002)
3.1.Oligonucleotide primers
Forward primer RS-1-F | 5′- ACT AAC GAA GCA GAG ATG CAT TA -3′ |
Reverse primer RS-1-R | 5′- CCC AGT CAC GGC AGA GAC T -3′ |
Forward primer NS-5-F | 5′- AAC TTA AAG GAA TTG ACG GAA G -3′ |
Reverse primer NS-6-R | 5′- GCA TCA CAG ACC TGT TAT TGC CTC -3′ |
Expected amplicon size from R. solanacearum template DNA = 718 bp (RS-primer set)
Expected amplicon size from the 18S rRNA internal PCR control = 310 bp (NS-primer set).
Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..