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a Method was validated using Taq polymerase from Perkin Elmer (AmpliTaq) and Gibco BRL.] | ||
Reagent | Quantity per reaction | Final concentration |
---|---|---|
Sterile UPW | 17,65 µl | |
10X PCR buffer a (15 mM MgCl 2 ) | 2,5 µl | 1X (1,5 mM MgCl 2 ) |
dNTP mix (20 mM) | 0,25 µl | 0,2 mM |
Primer OLI-1 (20 µM) | 1,25 µl | 1µM |
Primer Y-2 (20 µM) | 1,25 µl | 1µM |
Taq polymerase (5U/µl) a | 0,1 µl | 0,5 U |
Sample volume | 2,0 µl | |
Total volume | 25 µl |