[4. Buffers for DASI ELISA test U.K.
4.1. Coating buffer, pH 9,6 U.K.
| Na 2 CO 3 | 1,59 g |
| NaHCO 3 | 2,93 g |
| Distilled water | 1 000 ml |
Dissolve ingredients and check pH 9,6
4.2. 10X Phosphate saline buffer(PBS) pH 7,2 to 7,4 U.K.
| NaCl | 80,0 g |
| NaH 2 PO 4 .2 H 2 O | 4,0 g |
| Na 2 HPO 4 .12H 2 O | 27,0 g |
| Distilled water | 1 000 ml |
4.3. PBS-Tween U.K.
| 10X PBS | 50 ml |
| 10 % Tween 20 | 5 ml |
| Distilled water | 950 ml |
4.4. Substrate buffer, pH 9,8 U.K.
| Diethanolamine | 100 ml |
| Distilled water | 900 ml |
Mix and adjust to pH 9,8 with concentrated HCl.]
