Council Directive 98/57/ECShow full title

Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.

[F1Kelman’s Tetrazolium Medium U.K.
Casamino acids (Difco) 1,0 g
Bacto-Peptone (Difco) 10,0 g
Dextrose 5,0 g
Bacto-Agar (Difco) 15,0 g
Distilled water 1,0 l

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 minutes.

Cool to 50 °C and add a filter-sterilised solution of 2,3,5-triphenyl tetrazolium chloride (Sigma) to obtain a final concentration of 50 mg per l.]