Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot (c. 85)

[F1 ANNEX I U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. SCOPE OF THE TEST SCHEME

Textual Amendments

F1 Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot.

Appendix 5 Media for isolation and culture of C. m. subsp. sepedonicus

(a) General growth media U.K.

Nutrient agar (NA) U.K.

Nutrient Agar (Difco)	23,0 g
Distilled water	1,0 L

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 min.

Nutrient dextrose agar (NDA) U.K.

Difco bacto nutrient agar containing 1 % D(+) glucose (monohydrate). Sterilize by autoclaving at 115 °C for 20 minutes.

Yeast peptone glucose agar (YPGA) U.K.

Yeast Extract (Difco)	5,0 g
Bacto-Peptone (Difco)	5,0 g
D(+) Glucose (monohydrate)	10,0 g
Bacto-Agar (Difco)	15,0 g
Distilled water	1,0 L

Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 min.

Yeast extract mineral salts medium (YGM) U.K.

Bacto-Yeast-Extract (Difco)	2,0 g
D(+) Glucose (monohydrate)	2,5 g
K 2 HPO 4	0,25 g
KH 2 PO 4	0,25 g
MgSO 4 .7H 2 O	0,1 g
MnSO 4 .H 2 O	0,015 g
NaCl	0,05 g
FeSO 4 .7H 2 O	0,005 g
Bacto-Agar (Difco)	18 g
Distilled water	1,0 L

Dissolve ingredients and sterilize 0,5 litre volumes of medium by autoclaving at 115 °C for 20 minutes.]