Search Legislation

First Commission Directive of 25 October 1985 on methods of analysis for edible caseins and caseinates (85/503/EEC)

 Help about what version

What Version

 Help about advanced features

Advanced Features

Close

This is a legislation item that originated from the EU

After exit day there will be three versions of this legislation to consult for different purposes. The legislation.gov.uk version is the version that applies in the UK. The EU Version currently on EUR-lex is the version that currently applies in the EU i.e you may need this if you operate a business in the EU.

The web archive version is the official version of this legislation item as it stood on exit day before being published to legislation.gov.uk and any subsequent UK changes and effects applied. The web archive also captured associated case law and other language formats from EUR-Lex.

Changes over time for: METHOD 3

 Help about opening options

Status:

EU Directives are being published on this site to aid cross referencing from UK legislation. After exit day no further amendments will be applied to this version.

METHOD 3U.K.

DETERMINATION OF TITRATABLE ACIDITY U.K.

1.SCOPE AND FIELD OF APPLICATIONU.K.

The method determines the titratable acidity of:

  • acid caseins.

2.DEFINITIONU.K.

The titratable acidity of acid caseins: the volume in millilitres, of a 0,1 mol/1 standard sodium hydroxide solution required to neutralize an aqueous extract of 1 gram of the product.

3.PRINCIPLEU.K.

An aqueous extract of the sample at 60 o C is obtained and filtered. The filtrate is titrated against standard sodium hydroxide using phenolphtalein indicator.

4.REAGENTSU.K.

Any water used in the method procedure or in the preparation of reagents shall be freed from carbon dioxide by boiling for 10 minutes before use.

4.1. Sodium hydroxide solution: 0,1 Mol/1.U.K.
4.2. Phenolphtalein indicator solution, 10 g/1 in ethanol (95 % V/V) neutralized to the indicator.U.K.
5.APPARATUSU.K.
5.1. Analytical balance U.K.
5.2. Conical flask, 500 ml capacity, with ground neck and fitted with a ground glass stopper.U.K.
5.3. One-mark pipette, 100 ml capacity.U.K.
5.4. Pipette, suitable for measuring 0,5 ml of indicator solution (4.2).U.K.
5.5. Conical flask, 250 ml capacity.U.K.
5.6. Measuring cylinder, 250 ml capacity.U.K.
5.7. Burette, graduated in 0,1 ml.U.K.
5.8. Water bath, capable of being controlled at a temperature of 60 oC ± 2 oC.U.K.
5.9. Appropriate filter U.K.
6.PROCEDUREU.K.
6.1. Preparation of the test sample U.K.

As described in Section 1.2 of the General Provisions.

6.2. Test portion U.K.

Weigh about 10 grams of the test sample (6.1) to the nearest 10 mg and transfer it to the conical flask (5.2).

6.3. Determination U.K.

Using the 250 ml measuring cylinder (5.6), add 200 ml of freshly boiled and cooled water, previously heated to 60 o C. Stopper the flask, mix by swirling and place in the water bath at 60 o C (5.8) for 30 minutes. Shake the flask at intervals of about 10 minutes.

Filter, and cool the filtrate to about 20 oC. The filtrate must be clear.

Transfer 100 ml of the cooled filtrate into the conical flask (5.5), using the pipette (5.3). Add 0,5 ml of the phenolphtalein indicator solution (4.2), using the pipette (5.4). Titrate with the standard volumetric sodium hydroxide solution (4.1), until the appearance of a faint pink colour, persisting for at least 30 seconds. Determine and record the volume used to the nearest 0,01 ml.

7.EXPRESSION OF RESULTSU.K.
7.1. Formula and method of calculation U.K.

The titratable acidity of the acid casein is given by:

where:

V

is the volume, in millilitres, of the standard volumetric sodium hydroxide solution (4.1) used;

T

is the strength of the standard volumetric sodium hydroxide solution (4.1) in mol/1;

m

is the mass, in grams, of the test portion.

Calculate the titratable acidity to two decimal places.

7.2. Repeatability U.K.

The difference in results between two determinations carried out simultaneously or in rapid succession on the same sample, by the same analyst, under the same conditions, shall not exceed 0,02 ml of 0,1 mol/1 sodium hydroxide per 1 gram of product.

This repeatability interval should be achieved in 95 % of the times that the method is correctly carried out.

Back to top

Options/Help

Print Options

Close

Legislation is available in different versions:

Latest Available (revised):The latest available updated version of the legislation incorporating changes made by subsequent legislation and applied by our editorial team. Changes we have not yet applied to the text, can be found in the ‘Changes to Legislation’ area.

Original (As adopted by EU): The original version of the legislation as it stood when it was first adopted in the EU. No changes have been applied to the text.

Close

See additional information alongside the content

Geographical Extent: Indicates the geographical area that this provision applies to. For further information see ‘Frequently Asked Questions’.

Show Timeline of Changes: See how this legislation has or could change over time. Turning this feature on will show extra navigation options to go to these specific points in time. Return to the latest available version by using the controls above in the What Version box.

Close

Opening Options

Different options to open legislation in order to view more content on screen at once

Close

More Resources

Access essential accompanying documents and information for this legislation item from this tab. Dependent on the legislation item being viewed this may include:

  • the original print PDF of the as adopted version that was used for the EU Official Journal
  • lists of changes made by and/or affecting this legislation item
  • all formats of all associated documents
  • correction slips
  • links to related legislation and further information resources
Close

Timeline of Changes

Close

More Resources

Use this menu to access essential accompanying documents and information for this legislation item. Dependent on the legislation item being viewed this may include:

  • the original print PDF of the as adopted version that was used for the print copy
  • correction slips

Click 'View More' or select 'More Resources' tab for additional information including:

  • lists of changes made by and/or affecting this legislation item
  • confers power and blanket amendment details
  • all formats of all associated documents
  • links to related legislation and further information resources