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Commission Decision of 23 May 2005 on protection and surveillance zones in relation to bluetongue and conditions applying to movements from or through these zones (notified under document number C(2005) 1478) (Text with EEA relevance) (2005/393/EC) (repealed)

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ANNEX IIas referred to in Article 3(1)

A.Live animals must have been:

1.

protected from culicoïdes attack for at least 100 days prior to movement; or

2.

protected from culicoïdes attack for at least 28 days prior to movement, and subjected during that period to a serological test to detect antibody to the BTV group, such as the BT competition ELISA or the BT AGID test, with negative results on two occasions, with an interval of not less than seven days between each test, the first test being carried out at least 21 days after introduction into the quarantine station; or

3.

protected from culicoïdes attack for at least 14 days prior to movement, and subjected during that period to a BTV isolation test or polymerase chain reaction test, with negative results, on blood samples taken on two occasions, with an interval of not less than seven days between each test, the first test being carried out at least seven days from the date of introduction into the quarantine station; and

4.

protected from culicoïdes attack during transportation to the place of movement.

B.Semen must have been obtained from donors, which have been:

1.

protected from culicoïdes attack for at least 100 days before the date of commencement of, and during, collection of the semen; or

2.

subjected to a serological test to detect antibody to the BTV group such as the BT competition ELISA or the BT AGID test, with negative results, at least every 60 days throughout the collection period and between 28 and 60 days after the final collection for this consignment; or

3.

subjected to a virus isolation test or polymerase chain reaction (PCR) test on blood samples collected at commencement and conclusion of, and at least every seven days (virus isolation test) or at least every 28 days (PCR test) during, semen collection for this consignment, with negative results.

C.Ovas and embryos must have been obtained from donors, which have been:

1.

protected from culicoïdes attack for at least 100 days before date of commencement of and during, collection of the embryos/ovas; or

2.

subjected to a serological test to detect antibody to the BTV group such as the BT competition ELISA or the BT AGID test, between 28 and 60 days from the date of collection with negative results; or

3.

subjected to a BTV isolation test or polymerase chain reaction test on a blood sample taken on the day of collection, with negative results.

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