SCHEDULE 2Testing Methods

PART 1METHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS

Subcultured colonies

9.  After incubation each plate shall be examined for colonies characteristic of Clostridium perfringens. All colonies characteristic of Clostridium perfringens shall be–

(a)stab inoculated into motility nitrate medium(1); and

(b)inoculated into either lactose gelatin medium(2) or charcoal gelatin discs(3), and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

(1)

Motility nitrate medium – see Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884–892. National Research Council of Canada, Ottawa ON K1A oR6, Canada.

(2)

Lactose gelatin medium – see Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefield R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884–892.

(3)

Charcoal gelatin discs – see Mackie and McCartney, (1996) Practical Medical Microbiology 14, 509. Churchill Livingstone, Robert Stevenson House, 1-3 Baxter’s Place, Leith Walk, Edinburgh EH1 3AF.